
Article 1 
Rooster comb extract as defined and specified in Annex I may be placed on the market as a novel food ingredient for the uses defined and at the maximum levels established in Annex II.
Article 2 
The designation of rooster comb extract, authorised by this Decision on the labelling of the foodstuffs containing it shall be ‘rooster comb extract’ or ‘cockerel comb extract’.
Article 3 
This Decision is addressed to Bioiberica S.A., Plaça Francesc Macià, 7. 8B, 08029 Barcelona Spain.
Done at Brussels, 29 November 2013.
For the Commission
Tonio BORG
Member of the Commission
ANNEX I
Rooster comb extract is obtained from Gallus gallus by enzymatic hydrolysis of rooster comb and by subsequent filtration, concentration and precipitation steps. The principal constituents of rooster comb extract are the glycosaminoglycans hyaluronic acid, chondroitin sulphate A and dermatan sulphate (chondroitin sulphate B).


Hyaluronic acid 60-80 %
Chondroitin sulphate A Not more than 5 %
Dermatan sulphate (chondroitin sulphate B) Not more than 25 %

White or almost white hygroscopic powder.


pH 5,0-8,5


Chlorides Not more than 1 %
Nitrogen Not more than 8 %
Loss on drying (105 °C for 6 hours) Not more than 10 %
Mercury Not more than 0,1 mg/kg
Arsenic Not more than 1 mg/kg
Cadmium Not more than 1 mg/kg
Chromium Not more than 10 mg/kg
Lead Not more than 0,5 mg/kg


Total viable aerobic count Not more than 102 cfu/g
Escherichia coli Absent in 1 g
Salmonella spp. Absent in 1 g
Staphylococcus aureus Absent in 1 g
Pseudomonas aeruginosa Absent in 1 g

ANNEX II
Food category Maximum use level (mg/100 g or mg/100 ml)
Milk-based drinks 40
Milk based fermented drinks 80
Yoghurt-type products 65
Fromage frais 110