
Article 1 
The preparations belonging to the group ‘Micro-organisms’, as specified in Annex I, are authorised for use without a time limit as additives in animal nutrition under the conditions laid down in that Annex.
Article 2 
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Article 3 
The preparations belonging to the group ‘Enzymes’, as specified in Annex III, are authorised for use for four years as additives in animal nutrition under the conditions laid down in that Annex.
Article 4 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
ANNEX I

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
CFU/kg of complete feedingstuff
Micro-organisms



ANNEX II


ANNEX III

EC No or No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes


37 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Subtilisin
EC 3.4.21.62 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:
 Endo-1,4-beta-xylanase: 5 000 U/g
 Subtilisin: 1 600 U/g Ducks — endo-1,4-beta-xylanase:2 500 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 2 500 U
 subtilisin: 800 U.

3. For use in compound feed e.g. containing more than 65 % wheat.
 4.1.2010
subtilisin:800 U —
59 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Subtilisin
EC 3.4.21.62
 Alpha-amylase
EC 3.2.1.1
 Polygalacturonase
EC 3.2.1.15 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a mimimum activity of:
 Endo-1,4-beta-xylanase: 300 U/g
 Endo-1,3(4)-beta-glucanase: 150 U/g
 Subtilisin: 4 000 U/g
 Alpha-amylase: 400 U/g
 Polygalacturonase: 25 U/g Ducks — endo-1,4-beta-xylanase:300 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 300 U
 endo-1,3(4)-beta-glucanase: 150 U
 subtilisin: 4 000 U
 alpha-amylase: 400 U
 polygalacturonase: 25 U.

3. For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize.
 4.1.2010
endo-1,3(4)-beta-glucanase:150 U —
subtilisin:4 000 U —
alpha-amylase:400 U —
polygalacturonase:25 U —
Laying hens — endo-1,4-beta-xylanase:225 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 225 U
 endo-1,3(4)-beta-glucanase: 112 U
 subtilisin: 3 000 U
 alpha-amylase: 300 U
 polygalacturonase: 18 U.

3. For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize.
 4.1.2010
endo-1,3(4)-beta-glucanase:112 U —
subtilisin:3 000 U —
alpha-amylase:300 U —
polygalac-turonase:18 U —





