
Article 1 
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Article 2 
The conditions for the authorisation of the preparations No 16 and No 17 belonging to the group ‘Enzymes’ listed in Annex II to the present Regulation are hereby replaced by those set out in the said Annex according to Directive 70/524/EEC.
Article 3 
The preparations belonging to the group ‘Enzymes’ listed in Annex III to the present Regulation shall be authorised according to Directive 70/524/EEC as additives in animal nutrition under the conditions laid down in the said Annex.
Article 4 
The preparation belonging to the group ‘Micro-organisms’ listed in Annex IV to the present Regulation shall be authorised according to Directive 70/524/EEC as additives in animal nutrition under the conditions laid down in the said Annex.
Article 5 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Communities.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
ANNEX I


ANNEX II

No(or EC No) Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions Period of authorisation
Units of activity/kg of complete feedingstuff
16 Endo-1,4-beta-glucanaseEC 3.2.1.4 Preparation of endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (IMI SD 142) having a minimum activity of:
 Solid form: 2 000 CU/g
 Liquid form: 2 000 CU/ml Chickens for fattening — 250 CU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500-1 000 CU.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.
 30.9.2000
Laying hens — 250 CU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500-1 000 CU.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.
 30.9.2000
Piglets 4 months 250 CU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500-1 000 CU.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.
 30.9.2000
Pigs for fattening — 250 CU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500-1 000 CU.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.
 30.9.2000
17 Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:
 Solid form: 6 000 EPU/g
 Liquid form: 2 000 EPU/ml Chickens for fattening — 750 EPU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabi-noxylans), e.g. containing more than 40 % wheat.
 30.9.2000
Laying hens — 750 EPU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.
 30.9.2000
Piglets 4 months 750 EPU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.
 30.9.2000
Pigs for fattening — 750 EPU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.
 30.9.2000


ANNEX III

No.(or EC No) Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions Period of authorisation
Units of activity/kg of complete feedingstuff
12 Endo-1,4-beta-glucanaseEC 3.2.1.4 Preparation of endo-1,4-beta-glucanase, endo-1,3 (4)-beta-glucanase and endo-1,4-beta-xylanase produced by Trichoderma viride (FERM BP-4447) having a minimum activity
 Endo-1,4-beta-glucanase: 8 000 U/g
 Endo-1,3(4)-glucanase: 18 000 U/g
 Endo-1,4-beta-xylanase: 26 000 U/g Turkeys for fattening — Endo-1,4-beta-glucanase 800 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 Endo-1,4-beta-glucanase: 800-1 200 U
 Endo-1,3 (4)-beta-glucanase: 1 800-2 700 U
 Endo-1,4-beta-xylanase: 2 600-3 900 U.

3. For use in compound feed rich in non-strach polysaccarides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20 % wheat and 20 % barley.
 30.9.2001
Endo-1,3(4)-beta-glucanaseEC 3.2.1.6 Endo-1,3(4)-beta-glucanase: 1 800 U —
Endo-1,4-beta-xilanaseEC 3.2.1.8 Endo-1,4-beta-xylanase: 2 600 U —
17 Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:
 Solid form: 6 000 EPU/g
 Liquid form: 6 000 EPU/ml Turkeys for fattening — 750 EPU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 1 500-3 000 EPU.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 35 % wheat.
 30.9.2001
42 Endo-1,4(4)-betaxylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:
 Solid form: 4 000 U/gCharacteristic of the authorised preparation:
 endo-1,4-beta-xylanase: 1,99 %
 wheat: 97,7 %
 calcium propionate: 0,3 %
 lecithin: 0,01 % Pigs for fattening — 4 000 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 4 000 U

3. For use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 60 % wheat.
 30.9.2001
49 Endo-1,3(4)-beta-glucanaseEC 3.2.1.6 Preparation of endo-1,3(4)-beta-glucanase producted by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9554) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:
 endo-1,3(4)-beta-glucanase: 150 U/g
 endo-1,4-beta-xylanase: 1 500 U/g
 alfa-amylase: 500 U/g
 bacillolysin: 800 U/g
 polygalacturonase: 50 U/g Chickens for fattening — Endo-1,3(4)-beta-glucanase: 150 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 150 U
 endo-1,4-beta-xylanase: 1 500 U
 alpha-amylase: 800 U.
 bacillolysin: 800 U
 polygalacturonase: 50 U

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and betaglucans), e.g. containing more than 30 % wheat.
 30.9.2001
Endo-1,4-beta-xilanasaEC 3.2.1.8 Endo-1,4-beta-xylanase: 1 500 U —
Alfa-amylaseEC 3.2.1.1 Alfa-amylase: 500 U —
BacillolysinEC 3.4.24.28 Bacillolysin 800 U —
PolygalacturonaseC 3.2.1.15 Polygalacturonase 50 U —
 Layinghens — endo-1,3(4)-beta-glucanase: 150 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 150 U
 endo-1,4-beta-xylanase: 1 500 U
 alpha-amylase: 1 000 U
 polygalacturonase: 25 U.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and betaglucans), e.g. containing more than 30 % wheat.
 30.9.2001
 endo-1,4-beta-xylanase: 1 500 U —
 alpha-amylase: 1 000 U —
 bacillolysin: 800 U —
 polygalacturonase: 50 U —
50 6-phytaseEC 3.1.3.26 Preparation of 6-phytase produced by Aspergillus oryzae (DSM 11857) having a minimum activity of:
 Coated form: 2 500 FYT/g
 Liquid form: 5 000 FYT/g Chickens for fattening — 250 FYT — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 500-1 000 FYT

3. For use in compound feed containing more than 0,25 % phytin bound phosphorus.
 30.9.2001
Laying hens — 250 FYT — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 500-1 000 FYT

3. For use in compound feed containing more than 0,25 % phytin bound phosphorus.
 30.9.2001
Turkeys for fattening — 250 FYT — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 500-1 000 FYT

3. For use in compound feed containing more than 0,25 % phytin bound phosphorus.
 30.9.2001
Piglets 2 months 500 FYT — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 500-1 000 FYT

3. For use in compound feed containing more than 0,25 % phytin bound phosphorus.
 30.9.2001
Pigs for fattening — 500 FYT — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 500-1 000 FYT

3. For use in compound feed containing more than 0,25 % phytin bound phosphorus.
 30.9.2001
51 Endo-1,4-betaxylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG-S 15136) having a minimum activity of:
 100 IU/g Chickens for fattening — 10 IU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 10- IU

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.
 30.9.2001
52 Endo-1,3(4)-beta-glucanase:EC 3.2.1.6 Preparation of endo-1,3(4)-beta-glucanasa produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), having a minimum activity of:
 Liquid form:
 Endo-1,3 (4)-beta-glucanase: 10 000 U/m
 Endo-1,4-beta-glucanase: 120 000 U/m
 Alpha-amylase: 400 U/ml Chickens for fattening — Endo-1,3(4)-beta-glucanase: 1 000 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 1 000-2 000 U endo-1,4-beta-glucanase: 12 000-24 000 U

3. For use in compound feed rich in non starch polysaccharides (mainly arabinoxylans and betaglucans) e.g. containing more than 20 % wheat and 15 % sorghum and 5 % maize.
 30.9.2001
Endo-1,4-beta-glucanase:EC 3.2.1.4 Endo-1,4-beta-glucanase: 12 000 U —
Alpha-amylaseEC 3.2.2.1 Alpha-amylase: 40 U —















ANNEX IV

No. Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions Period of authorisation
CFU/kg of complete feedingstuff
19 Streptococcus infantariusCNCM I-841 Mixture of:Streptococcus infantarius and Lactobacillus plantarum containing a minimum of:Streptococcus infantarius0,5 × 109 CFU/gand:Lactobacillus plantarum2 × 109 CFU/g Calves 6 months Streptococcus infantarius1 × 109 Streptococcus infantarius1 × 109 In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting. 30.9.2001
Lactobacillus plantarumCNCM I-840 Lactobacillus plantarum:0,5 × 109 Lactobacillus plantarum:0,5 × 109