
Article 1 
The substance specified in the Annex, belonging to the additive category ‘sensory additives’ and to the functional group ‘colourants’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.
Article 2 

1. The substance specified in the Annex and premixtures containing that substance, which are produced and labelled before 27 August 2020 in accordance with the rules applicable before 27 February 2020 may continue to be placed on the market and used until the existing stocks are exhausted.
2. Feed materials and compound feed containing the substance specified in the Annex which are produced and labelled before 27 February 2022 in accordance with the rules applicable before 27 February 2020 may continue to be placed on the market and used until the existing stocks are exhausted.
Article 3 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 6 February 2020.
For the Commission
The President
Ursula VON DER LEYEN
ANNEX

Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg of active substance of kg of complete feedingstuff with a moisture content of 12 %
Category: Sensory additives. Functional group: Colourants. (i) substances that add or restore colour in feedingstuffs
2a133 Brilliant Blue FCF Additive composition:Brilliant Blue FCF described as the sodium salt as the principal component.Solid form (powder)————————————Characterisation of the active substance as the sodium salt:Disodium α-(4-(N-ethyl-3-sulphonatobenzylamino) phenyl)-α-(4-N-ethyl-3-sulphonatobenzylamino) cyclohexa-2,5-dienylidene) toluene-2- sulphonateThe calcium and the potassium salts are also permitted.Chemical formula: C37H34N2Na2O9S3Solid form (powder) produced by chemical synthesisCAS number: 3844-45-9Purity criteriaContent not less than 85 % total colouring matters, calculated as the sodium salt (assay)Water insoluble matter: ≤ 0,2 %Subsidiary colouring matters: ≤ 6 %Organic compounds other thancolouring matters:
— Sum of 2-, 3- and 4-formyl benzene sulfonic acids: ≤ 1,5 %
— 3-((ethyl)(4-sulfophenyl) amino) methyl benzene sulfonic acid: ≤ 0,3 %Leuco base: ≤ 5 %Unsulfonated primary aromatic amines: ≤ 0,01 % (calculated as aniline)Ether extractable matter: ≤ 0,2 % from a solution of pH 7————————————Analytical methodFor the quantification of total colouring matters content of brilliant blue FCF in the feed additive:Spectrophotometry at 630 nm and titration with Titanous chloride as described in:
— Commission Regulation (EU) No 231/2012 referring to FAO JECFA Combined Compendium for Food Additive Specifications (Analytical methods Vol. 4); and the Monograph No 1 (2006) ‘Brilliant Blue FCF’.For the quantification of brilliant blue in feedingstuffs:
— high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) Cats — — 278 
1.. In the directions for use of the additive and premixture, the storage conditions and stability to heat treatment shall be indicated.
2.. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including eye, skin and breathing protection. 27 February 2030
Dogs — — 334
