
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘other zootechnical additives’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 5 February 2020.
For the Commission
The President
Ursula VON DER LEYEN
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Unit of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: other zootechnical additives.
4d16 DSM Nutritional Products Ltd. represented in the Union by DSM Nutritional Products Sp. Z o.o Muramidase (EC 3.2.1.17) Additive compositionPreparation of muramidase (EC 3.2.1.17) (lysozyme) produced by Trichoderma reesei (DSM 32338) having a minimum activity of 60 000 LSU(F) /gSolid and liquid form Chickens reared for breedingTurkeys for fattening — 25 000LSU(F)  
1.. In the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment shall be indicated.
2.. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin and breathing protections. 26 February 2030
Characterisation of the active substance:muramidase (EC 3.2.1.17) (lysozyme) produced by Trichoderma reesei (DSM 32338) Turkeys reared for breeding
Analytical methodFor the quantification of muramidase:fluorescence-based enzyme assay method that determines the enzyme-catalyzed depolymerisation of a fluorescein-labelled peptidoglycan preparation at pH 6,0 and 30 °C. Other poultry species reared for breeding

