
Article 1 
The substance specified in the Annex, belonging to the additive category ‘nutritional additives’ and to the functional group ‘amino acids, their salts and analogues’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 1 October 2020.
For the Commission
The President
Ursula VON DER LEYEN
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method. Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg/kg of complete feed with a moisture content of 12 %
Category of nutritional additives. Functional group: amino acids, their salts and analogues.
3c441 - L-tryptophan Additive composition:Powder with a minimum of 98 % L-tryptophan (on a dry matter basis)Maximum content of 10 mg/kg 1,1′-ethylidene-bis-L-tryptophan (EBT) All species - - - 
1.. L-tryptophan may be placed on the market and used as an additive consisting of a preparation.
2.. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks by inhalation, dermal contact or eyes contact. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing protection, safety glasses and gloves.
3.. The endotoxin content of the additive and its dusting potential shall ensure a maximal endotoxin exposure of 1600 IU endotoxins/m3 air.
4.. L-tryptophan may be used via water for drinking.
5.. For ruminants, L-tryptophan shall be rumen protected.
6.. The labelling of the additive and premixtures shall indicate the following:
'
 The supplementation with L- tryptophan, in particular via water for drinking, shall take into account all essential and conditionally essential amino acids in order to avoid imbalances.'
7.. Declarations to be made on the label of the additive: Moisture content. 22.10.2030
Characterisation of the active substance:L-tryptophan produced by fermentation with Escherichia coli CGMCC 7.267 or Escherichia coli KCCM 10 534 orEscherichia coli CGMCC 11674Chemical formula: C11H12N2O2CAS No: 73-22-3
Analytical methods:For the identification of L-tryptophan in the feed additive:
— Food Chemical Codex ‘L-tryptophan monograph’.For the determination of tryptophan in the feed additive and premixtures:
— High performance liquid chromatography with fluorescence detection (HPLC-FLD) – EN ISO 13 904For the determination of tryptophan in compound feed and feed materials:
— High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD) – Commission Regulation (EC) No 152/2009 (Annex III, G)For the determination of tryptophan in water:
— High performance liquid chromatography with fluorescence detection (HPLC-FLD)

