
Article 1 
The substance specified in the Annex, belonging to the additive category ‘nutritional additives’ and to the functional group ‘amino acids, their salts and analogues’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 28 May 2019.
For the Commission
The President
Jean-Claude JUNCKER
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method. Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg/kg of complete feed with a moisture content of 12 %
Category of nutritional additives. Functional group: amino acids, their salts and analogues.
3c410 — L-threonine 
Powder with a minimum of 98 % L-threonine (on a dry matter basis).

L-threonine produced by fermentation with


 Escherichia coli CGMCC 7.232
 Chemical formula: C4H9NO3
 CAS Number: 72-19-5.

For the determination of L-threonine in the feed additive:


— Food Chemical Codex ‘L-threonine monograph’ and
— Ion exchange chromatography coupled with post-column derivatisation and optical detection (IEC-VIS/FD) – EN ISO 17180.

For the determination of threonine in premixtures:


— ion exchange chromatography coupled with post-column derivatisation and optical detection (IEC-VIS/FD) – EN ISO 17180 and
— ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-VIS), Commission Regulation (EC) No 152/2009 (Annex III, F).

For the determination of threonine in compound feed and feed materials:


— Ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-VIS): Commission Regulation (EC) No 152/2009 (Annex III, F).

For the determination of threonine in water:


— ion exchange chromatography coupled with post-column derivatisation and optical detection (IEC-VIS/FD).
 All species — — — 
1. L- threonine may be placed on the market and used as an additive consisting of a preparation.

2. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks by inhalation. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing protection.

3. The endotoxin content of the additive and its dusting potential shall ensure a maximal endotoxin exposure of 1 600 IU endotoxins/m3 air.

4. L-threonine may be used via water for drinking.

5. The labelling of the additive shall indicate the moisture content.

6. 
'If the additive is administered via water for drinking, protein excess should be avoided.'
 18 June 2029

