
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 8 March 2016.
For the Commission
The President
Jean-Claude JUNCKER
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a23 Lohmann Animal Nutrition GmbH 6-phytaseEC 3.1.3.26 
Preparation of 6-phytase produced by Komagataella pastoris (DSM 25375) having a minimum activity of: 40 000 U/g

Liquid and solid form

6-phytase (EC 3.1.3.26) produced by Komagataella pastoris (DSM 25375)

For the quantification of 6-phytase activity in the feed additive:

colorimetric method based on the enzymatic reaction of phytase on the phytate — VDLUFA Method Book, Vol. III, 27.1.1

For the quantification of 6-phytase activity in premixtures and mineral feeds:

colorimetric method based on the enzymatic reaction of phytase on the phytate — VDLUFA Method Book, Vol. III, 27.1.3

For the quantification of 6-phytase activity in feedingstuffs:

colorimetric method based on the enzymatic reaction of phytase on the phytate — EN ISO 30024.
 Chickens for fattening and reared for laying, all avian species for fattening and reared for laying other than turkeys for fattening and reared for breeding — 250 U — 
1. In the directions for use of the additive and premixture, indicate the storage conditions and stability to heating treatment.

2. For safety: breathing protection, safety glasses and gloves shall be used during handling.
 29 March 2026
All avian species for laying 125 U
Turkeys for fattening and reared for breeding 500 U
Piglets (weaned), pigs for fattening, sows and minor porcine species 250 U

