
Article 1 
The substance specified in the Annex, belonging to the additive category ‘nutritional additives’ and to the functional group ‘amino acids, their salts and analogues’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 

1. L-threonine authorised by Directive 88/485/EEC and premixtures containing it may be placed on the market until 16 May 2017 in accordance with the rules applicable before 16 August 2016 and used until the existing stocks are exhausted.
2. Feed materials and compound feed containing the substance referred to in paragraph 1 may be placed on the market until 16 August 2017 in accordance with the rules applicable before 16 August 2016 and used until the existing stocks are exhausted if they are intended for food producing animals.
3. Feed materials and compound feed containing the substance referred to in paragraph 1 may be placed on the market until 16 August 2018 in accordance with the rules applicable before 16 August 2016 and used until the existing stocks are exhausted if they are intended for non-food producing animals.
Article 3 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 26 July 2016.
For the Commission
The President
Jean-Claude JUNCKER
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method. Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg/kg of complete feed with a moisture content of 12 %
Category of nutritional additives. Functional group: amino acids, their salts and analogues.
3c410 — L-threonine 
Powder with a minimum of 98 % L-threonine (on a dry matter basis).

L-threonine produced by fermentation with Escherichia coli DSM 25086 or

Escherichia coli FERM BP-11383 or

Escherichia coli FERM BP-10942 or

Escherichia coli NRRL B-30843 or

Escherichia coli KCCM 11133P or

Escherichia coli DSM 25085 or

Escherichia coli CGMCC 3703 or

Escherichia coli CGMCC 7.58.

Chemical formula: C4H9NO3

CAS Number: 72-19-5

For the determination of L-threonine in the feed additive:


— Food Chemical Codex ‘L-threonine monograph’ and
— Ion exchange chromatography method with post-column derivatisation and photometric detection (IEC-UV/FD) — EN ISO 17180.

For the determination of threonine in premixtures:


— ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-UV/FD) — EN ISO 17180 and
— ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-UV), Commission Regulation (EC) No 152/2009 (Annex III, F)

For the determination of threonine in premixtures, compound feed, feed materials and water:


— Ion exchange chromatography method with post-column derivatisation and photometric detection (IEC-UV): Regulation (EC) No 152/2009 (Annex III, F).
 All species — — — 
1.. L- threonine may be placed on the market and used as an additive consisting of a preparation.
2.. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks by inhalation. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing protection.
3.. L-threonine can be also used via water for drinking.
4.. Declarations to be made on the labelling of the additive:
Moisture content.
5.. Declarations to be made on the labelling of the additive and premixtures:
'If the additive is administered via water for drinking, protein excess should be avoided.' 16.8.2026

