
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘other zootechnical additives’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 25 August 2015.
For the Commission
The President
Jean-Claude JUNCKER
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: other zootechnical additives (improvement of zootechnical parameters).
4d10 DSM Nutritional Products AG represented in the EU by DSM Nutritional Products Sp. z o.o. Poland Preparation of benzoic acid, thymol, eugenol, piperine 
Preparation of benzoic acid, thymol, eugenol, piperine having a content of:


— benzoic acid 80 %-83 %
— thymol 1 %-1,9 %
— eugenol 0,5 %-1 %
— piperine 0,05 %-0,1 %
— benzyl salicylate, iso-amyl salicylate and trans-anethole ≤ 0,6 %

benzoic acid (≥ 95,5 % purity): C7H6O2 CAS number: 65-85-0

thymol: C10H14O CAS number 89-83-8

eugenol: C10H12O2 CAS number 97-53-0

piperine: C17H19O3N CAS number 94-62-2

Isoamyl salicylate CAS number 87-20-7

Benzyl salicylate CAS number 118-58-1

Trans-anethole CAS number 4180-23-8

For the determination of benzoic acid in the feed additive and premixtures:


— Reverse Phase High-Performance Liquid Chromatography coupled to an Ultraviolet detector (RP-HPLC-UV)

For the determination of benzoic acid in compound feed:


— Gas Chromatography Isotope Dilution Mass Spectrometry (GC-IDMS)

For the determination of thymol, eugenol and piperine in the feed additive:


— Gas Chromatography coupled to a Flame Ionisation Detector (GC-FID)
 Chickens for fatteningChickens reared for layingMinor poultry species for fattening and reared for laying — — 300 
1. In the directions for use of the additive and premixture, indicate the storage conditions and stability to pelleting.

2. The additive shall not be used with other sources of benzoic acid or benzoates.

3. For safety: breathing protection, glasses and gloves shall be used during handling.

4. Minimum recommended dose: 300 mg/kg of complete feedingstuff
 15.9.2025

