
Article 1 
The substance specified in the Annex, belonging to the additive category ‘sensory additives’ and to the functional group ‘colorants, substances that add or restore colour in feedingstuffs’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
In Annex II to Regulation (EC) No 358/2005 the entry of the row E 131 is deleted.
Article 3 
The substance specified in the Annex and feed containing that substance, which are produced and labelled before 25 July 2015 in accordance with the rules applicable before 25 July 2013, may continue to be placed on the market and used until the existing stocks are exhausted.
Article 4 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 4 July 2013.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg/kg of complete feedingstuff with a moisture content of 12 %
Category of sensory additives. Functional group: colorants, substances that add or restore colour in feedingstuffs
2a131 — Patent Blue V 
 Active substance
Patent Blue V
 Characterisation of the active substance
Name: calcium or sodium compound of [4-(α-(4-diethylaminophenyl)-5-hydroxy-2,4-disulfophenyl-methylidene) 2,5-cyclohexadien-1-ylidene] diethylammonium hydroxide inner salt and subsidiary colouring matters together with sodium chloride and/or sodium sulphate and/or calcium sulphate as the principal uncoloured components. The potassium salt is also permitted.
 Additive composition
Purity criteria: minimum of 90 % of total colouring matters, calculated as the sodium, calcium or potassium salts.
Leuco base: not more than 1,0 %.
 Method of Analysis
— For the quantification of total colouring matters content of Patent Blue V in the feed additive and feedingstuffs: spectrophotometry at 638 nm (JECFA monograph No 1, Vol. 4 method recommended by Commission Directive 2008/128/EC). All non-food producing animals — — 250 For user safety: breathing protection, safety glasses and gloves should be worn during handling. 25 July 2023

