
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.
Article 2 
Regulation (EC) No 1259/2004 is amended as follows:

1.. Article 2 is replaced by the following:
'
Article 2 
The preparations belonging to the group “Enzymes”, as set out in Annexes III, IV, V and VI are authorised for use without a time limit as additives in animal nutrition under the conditions laid down in those Annexes.';
2.. Annex II is deleted.
Article 3 
In the Annex to Regulation (EC) No 1206/2005 all the data contained in the entry E1602 are deleted.
Article 4 
Regulation (EC) No 1876/2006 is amended as follows:

1.. Article 3 is deleted;
2.. Annex III is deleted.
Article 5 
The preparation specified in the Annex and feed containing that preparation, which are produced and labelled before 23 November 2013 in accordance with the rules applicable before 23 May 2013, may continue to be placed on the market and used until the existing stocks are exhausted.
Article 6 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 2 May 2013.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a1602i DSM Nutritional Products Endo-1,4-beta-xylanaseEC 3.2.1.8Endo-1,3(4)- beta-glucanaseEC 3.2.1.6Endo-1,4-beta-glucanaseEC 3.2.1.4 
 Additive composition
Preparation of endo-1,4-beta-xylanase, endo-1,3(4)-beta-glucanase and endo-1,4-beta-glucanase, produced by Trichoderma reesei (ATCC 74444) having a minimum activity of:

 endo-1,4-beta-xylanase 2 700 U/ml or g additive
 endo-1,3(4)-beta-glucanase 700 U/ml or g additive
 endo-1,4-beta-glucanase 800 U/ml or g additive(Liquid and solid form)
 Characterisation of the active substance
endo-1,4-betaxylanase, endo-1,4-betaglucanase and endo-1,3(4)-betaglucanase, produced by Trichoderma reesei (ATCC 74444)
 Analytical method
Characterisation of the active substances in the feedingstuff:
— Colorimetric method measuring water soluble dye released by the action of endo-1,4-beta-xylanase from cross-linked birchwood azoxylan substrate
— Colorimetric method measuring water soluble dye released by the action of endo-1,3(4)-beta-glucanase from cross-linked azobarley glucan substrate
— Colorimetric method measuring water soluble dye released by the action of endo-1,4-beta-glucanase from cross-linked azocarboxymethylcellulose substrate. Poultry for fattening other than turkeys for fattening — endo-1,4-beta-xylanase: 135 Uendo-1,3(4)-beta-glucanase: 35 Uendo-1,4-beta-glucanase: 40 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. For use in feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans).

3. For use in weaned piglets up to 35 kg.

4. For safety: breathing protection and gloves shall be used during handling.
 23 May 2023
Poultry for laying endo-1,4-beta-xylanase: 216 Uendo-1,3(4)-beta-glucanase: 56 Uendo-1,4-beta-glucanase: 64 U
Turkeys for fatteningPiglets(weaned) endo-1,4-beta-xylanase: 270 Uendo-1,3(4)-beta-glucanase: 70 Uendo-1,4-beta-glucanase: 80 U



