
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 18 December 2013.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a17 Kerry Ingredients and Flavours Alpha-galactosidase EC 3.2.1.22Endo-1,4-beta-glucanase EC 3.2.1.4 
 Additive composition
Preparation of alpha-galactosidase produced by Saccharomyces cerevisiae (CBS 615.94) and endo-1,4-beta-glucanase produced by Aspergillus niger (CBS 120604) having a minimum activity of:

— 1 000 U alpha-galactosidase/g,
— 5 700 U endo-1,4-beta-glucanase/g.Solid form
 Characterisation of the active substance
alpha-galactosidase (EC 3.2.1.22) produced by Saccharomyces cerevisiae (CBS 615.94) and endo-1,4-beta-glucanase (EC 3.2.1.4) produced by Aspergillus niger (CBS 120604).
 Analytical method
For the determination of:
— alpha-galactosidase: colorimetric method measuring p-nitrophenol released by action of alpha-galactosidase from p-nitrophenyl-alpha-galactopyranoside substrate,
— endo-1,4-beta-glucanase: colorimetric method measuring water soluble dye released by action of endo-1,4-beta-glucanase from azurine-crosslinked barley glucan substrate. Minor poultry species for fattening.Chickens reared for laying. — alpha-galactosidase50 Uendo-1,4-beta-glucanase 285 U — 
1.. In the directions for use of the additive and premixture, indicate the storage conditions and stability to pelleting.
2.. Maximum recommended dose:

— 100 U alpha-galactosidase/kg of complete feed,
— 570 U endo-1,4-beta-glucanase/kg of complete feed.
3.. For safety: breathing protection, glasses and gloves shall be used during handling. 8 January 2024


