
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 18 September 2012.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a1640 Danisco Animal Nutrition (legal entity Danisco (UK) Limited) 6-phytase EC 3.1.3.26 
 Additive composition
Preparation of 6-phytase (EC 3.1.3.26) produced by Schizosaccharomyces pombe (ATCC 5233) having a minimum activity of:
Liquid and solid form: 5 000 FTU/g
 Characterisation of the active substance
6-phytase (EC 3.1.3.26) produced by Schizosaccharomyces pombe (ATCC 5233)
 Analytical method
 Determination of 6-phytase EC 3.1.3.26 in feed additive: colorimetric method based on the quantification of inorganic phosphate released by the enzyme from sodium phytate.
 Determination of 6-phytase EC 3.1.3.26 in feed premixtures and feedingstuff: EN ISO 30024: colorimetric method based on the quantification of inorganic phosphate released by the enzyme from sodium phytate (after dilution with heat-treated whole grain flour). All avian species for fattening other than chickens for fattening, turkeys for fattening and ducks for fattening — 250 FTU  
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. For use in compound feed containing more than 0,23 % phytin-bound phosphorus.

3. Maximum recommended dose: 1 000 FTU/kg of complete feedingstuff.

4. For safety: breathing protection glasses and gloves shall be used during handling.
 9 October 2022
All avian species for laying other than laying hens 150 FTU

