
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 30 May 2011.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a 14 Aveve NV 
 Endo-1,4-β-xylanase
EC 3.2.1.8
 Endo-1,3(4)-β-glucanase
EC 3.2.1.6
 Polygalacturonase
EC 3.2.1.15 
 Additive composition
Preparation of endo-1,4-β-xylanase (EC 3.2.1.8) produced by Trichoderma reesei (MUCL 49755), endo-1,3(4)-β-glucanase (EC 3.2.1.6) produced by Trichoderma reesei (MUCL 49754) and polygalacturonase (EC 3.2.1.15) produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:
 solid form:
 Endo-1,4-β-xylanase: 21 400 XU/g
 Endo-1,3(4)-β-glucanase: 12 300 BGU/g
 Polygalacturonase: 460 PGLU/g.
 liquid form:
 Endo-1,4-β-xylanase: 10 700 XU/g
 Endo-1,3(4)-β-glucanase: 6 150 BGU/g
 Polygalacturonase: 230 PGLU/g.
 Characterisation of the active substance
Endo-1,4-β-xylanase (EC 3.2.1.8)
produced by Trichoderma reesei, endo-1,3(4)-β-glucanase (EC 3.2.1.6) produced by Trichoderma reesei and polygalacturonase (EC 3.2.1.15) produced by Aspergillus aculeatus
 Method of Analysis
Characterisation of the active substances in the additive and feedingstuffs:
— colorimetric method measuring water soluble dye released by action of endo-1,4-β-xylanase from dye cross-linked wheat arabinoxylan substrate,
— colorimetric method measuring water soluble dye released by action of endo-1,3(4)-β-glucanase from dye cross-linked barley betaglucan substrate,
— viscosimetric method based on a decrease of viscosity produced by action of polygalacturonase on the pectin-containing substrate, polymethylgalacturonic acid. Piglets (weaned)  
 Endo-1,4-β-xylanase: 2 140 XU
 Endo-1,3(4)-β-glucanase: 1 230 BGU
 Polygalacturonase: 46 PGLU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. For piglets (weaned) up to 35 kg.

3. For use in compound feed rich in non-starch polysaccharides.
 20 June 2021



