
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 7 April 2011.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a15 Danisco Animal Nutrition 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6 
 Additive composition
Preparation (solid and liquid form) of endo-1,4-beta-xylanase produced by Trichoderma reesei (ATCC PTA 5588) and endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (ATCC SD 2106) having respectively a minimum activity of 12 200 U/g and 1 520 U/g
 Characterisation of the active substance
Endo-1,4-beta-xylanase produced by Trichoderma reesei (ATCC PTA 5588) and endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (ATCC SD 2106)
 Analytical methods
Characterisation of the active substance in the additive, premixtures and feedingstuffs:
— colorimetric method measuring water soluble dye released by action of endo-1,4-β-xylanase from azurine cross-linked wheat arabinoxylan substrates,
— colorimetric method measuring water soluble dye released by action of endo-1,3(4)-β-glucanase from azurine cross-linked barley β-glucan substrates. Turkeys for fattening and reared for breedingLaying hens — 
 endo-1,4-beta-xylanase
1 220 U
 endo-1,3(4)-beta-glucanase
152 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. For use in feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30 % wheat, barley, rye and/or triticale.

3. For safety reasons: breathing protection, glasses and gloves shall be used during handling.

4. For piglets (weaned) up to 35 kg.
 28 April 2021
Other poultryPiglets (weaned)Pigs for fattening endo-1,4-beta-xylanase610 Uendo-1,3(4)-beta-glucanase76 U


