
Article 1 
The Annex to Regulation (EC) No 1091/2009 is replaced by the text in the Annex to this Regulation.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 7 April 2011.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a9 Aveve NV 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6 
 Additive composition
Preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (MUCL 49755) and endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (MUCL 49754)
having a minimum activity of 40 000 XU/g and 9 000 BGU/g
 Characterisation of the active substance
endo-1,4-beta-xylanase produced by Trichoderma reesei (MUCL 49755) and endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (MUCL 49754)
 Analytical method
 Characterisation of the active substance in the additive:
— colorimetric method based on reaction of dinitrosalicylic acid on reducing sugar produced by action of endo-1,4-β-xylanase on a xylan containing substrate,
— colorimetric method based on reaction of dinitrosalicylic acid on reducing sugar produced by action of endo-1,3(4)-β-glucanase on a β-glucan containing substrate.
 Characterisation of the active substances in the feedingstuffs:
— colorimetric method measuring water soluble dye released by action of endo-1,4-beta-xylanase from dye cross-linked wheat arabinoxylan substrate,
— colorimetric method measuring water soluble dye released by action of endo-1,3(4)-beta-glucanase from dye cross-linked barley beta-glucan substrate. Chickens for fattening — 3 000 XU675 BGU  
1. In the directions for use of the additive and premixture indicate the storage temperature, storage life, and stability to pelleting.

2. For use in feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30 % wheat, barley, rye and/or triticale.

3. For safety reasons: breathing protection, glasses and gloves shall be used during handling.
 4 December 2019
