
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘gut flora stabilisers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 25 February 2011.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
CFU/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: gut flora stabilisers
4b1820 Calpis Co. Ltd Japan, represented by Calpis Co. Ltd Europe Representative Office, France Bacillus subtilis C-3102 (DSM 15544) 
 Additive composition
Preparation of Bacillus subtilis C-3102 DSM 15544 containing a minimum of 1 × 1010 CFU/g
 Characterisation of active substance
Viable spores of Bacillus subtilis C-3102 (DSM 15544)
 Analytical methods
 Enumeration: spread plate method using tryptone soya agar with preheat treatment of feed samples
 Identification: Pulsed Field Gel Electrophoresis (PFGE) Chickens reared for laying — 5 × 108 — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. For safety: breathing protection, glasses and gloves shall be used during handling.

3. May be used in feed containing the following coccidiostats where authorised: decoquinate, monensin sodium, robenidine hydrochloride, diclazuril, lasalocid sodium, halofuginone, narasin, salinomycin sodium, maduramycin ammonium, narasin-nicarbazin, semduramycin sodium or nicarbazin.
 18 March 2021
Turkeys, minor avian species and other ornamental and game birds 3 × 108
