
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 23 December 2009.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a13 DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp.Z.o.o. Serine proteaseEC 3.4.21.- Additive composition:
 Preparation of serine protease (EC 3.4.21.-)
 produced by Bacillus licheniformis (DSM 19670) having a minimum activity of 75 000 PROT/gCharacterisation of the active substance:
 Serine protease (EC 3.4.21.-)
 produced by Bacillus licheniformis (DSM 19670)Analytical method:Colorimetric method measuring yellow complex para-nitroaniline (pNA) released by the enzyme from ‘Suc-Ala-Ala-Pro-Phe-pNA’ at pH 9,0 and at 37 °C Chickens for fattening — 15 000 PROT — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. For safety reasons: breathing protection, glasses and gloves shall be used during handling.
 13.1.2020

