
Article 1 
The preparation belonging to the group ‘Enzymes’, as specified in the Annex, is authorised without a time limit as additive in animal nutrition under the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 15 June 2010.
For the Commission
The President
José Manuel BARROSO
ANNEX

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1621 Endo-1,3(4)-beta-glucanaseEC 3.2.1.6Endo-1,4-beta-glucanaseEC 3.2.1.4Alpha-amylaseEC 3.2.1.1Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of:
 endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus
(CBS 589.94),
 endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum
(CBS 592.94),
 alpha-amylase produced by Bacillus amyloliquefaciens
(DSM 9553) and
 endo-1,4-beta-xylanase produced by Trichoderma viride
(NIBH FERM BP4842)
having a minimum activity of:
 Endo-1,3(4)-beta-glucanase: 10 000 U/g,
 Endo-1,4-beta-glucanase: 120 000 U/g,
 alpha-amylase: 400 U/g,
 Endo-1,4-beta-xylanase: 210 000 U/g. Laying hens — endo-1,3(4)-beta-glucanase500 U  
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 500-1 500 U
 endo-1,4-beta-glucanase: 6 000-18 000 U
 alpha-amylase: 20-60 U
 endo-1,4-beta-xylanase: 10 500-31 500 U.

3. For use in compound feed rich in non-starch polysaccharides, (mainly betaglucans and arabinoxylans), e.g. containing 30-50 % wheat.
 Without a time limit.
endo-1,4-beta-glucanase6 000 U
alpha-amylase20 U
endo-1,4-beta-xylanase10 500 U



