
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘nutritional additives’ and to the functional group ‘amino acids, their salts and analogues’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 23 April 2010.
For the Commission
The President
José Manuel BARROSO
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg/kg of complete feedingstuff with a moisture content of 12 %
Category of nutritional additives. Functional group: amino acids, their salts and analogues
3c3.8.1 — L-isoleucine 
 Additive composition:
 L-isoleucine with a purity of at least 93,4 % (on dry matter) produced by Escherichia coli (FERM ABP-10641)
 ≤ 1 % unidentified impurities (as dry matter)
 Characterisation of the active substance:
L-isoleucine (C6H13NO2)
 Analytical method:
Method for the determination of amino acids as set out in Commission Regulation (EC) No 152/2009 All animal species — — — 
1. The moisture content shall be indicated.

2. For safety reasons: breathing protection shall be used during handling.
 14 May 2020
