
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 2 April 2009.
For the Commission
Androulla VASSILIOU
Member of the Commission
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers.
4a7 BASF SE Endo-1,4-beta-xylanaseEC 3.2.1.8Endo-1,4-beta-glucanaseEC 3.2.1.4 
 Additive composition:
Preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713)
and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404) having a minimum activity of:
 Solid form:
5 600 TXU and 2 500 TGU/g
 Liquid form:
5 600 TXU and 2 500 TGU/g
 Characterisation of the active substance:
endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404)
 Analytical method
For quantification of endo-1,4-beta-xylanase activity:
viscosimetric method based on decrease of viscosity produced by action of endo-1,4-beta-xylanase on the xylan containing substrate (wheat arabinoxylan) at pH = 3,5 and 55 °C.
For quantification of endo-1,4-beta-glucanase activity:
viscosimetric method based on decrease of viscosity produced by action of endo-1,4-beta-glucanase on the glucan containing substrate (barley betaglucan) at pH = 3,5 and 40 °C. Piglets(weaned) - 560 TXT250 TGU - 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. For use in feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e. g. containing more than 30 % wheat, barley, rye and/or triticale.

3. 

 Piglets (weaned):
560-840 TXU/250-375 TGU;
 Chickens for fattening:
280-840 TXU/125-375 TGU;
 Laying hens:
560-840 TXU/250-375 TGU;
 Turkeys for fattening:
560-840 TXU/250-375 TGU;
 Ducks for fattening:
280-840 TXU/125-375 TGU.

4. For use in weaned piglets until approximately 35 kg.

5. For safety: breathing protection, glasses and gloves shall be used during handling.
 22 April 2019
Chickens for fattening 280 TXT125 TGU
Laying hens 560 TXT250 TGU
Turkeys for fattening 560 TXT250 TGU
Ducks for fattening 280 TXT125 TGU


