
Article 1 
The preparation belonging to the group ‘Enzymes’, as specified in Annex I, is authorised without a time limit as additive in animal nutrition under the conditions laid down in that Annex.
Article 2 
The preparation belonging to the group ‘Enzymes’, as specified in Annex II, is authorised without a time limit as additive in animal nutrition under the conditions laid down in that Annex.
Article 3 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 21 December 2009.
For the Commission
The President
José Manuel BARROSO
ANNEX I

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1636 Endo-1,3(4)-beta-glucanaseEC 3.2.1.6 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:Solid form: 700 000 BU/gLiquid form: 300 000 BU/g Piglets (weaned) — 17 500 BU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. For use in weaned piglets up to 35 kg.

3. Recommended dose per kilogram of complete feedingstuff: 17 500-50 000 BU.

4. For use in compound feed rich in non-starch polysaccharides (mainly glucans), e.g. containing more than 60 % barley or wheat.
 Without a time limit

ANNEX II

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1620 Endo-1,3(4)-beta-glucanase EC 3.2.1.6Endo-1,4-beta-glucanase EC 3.2.1.4Alpha-amylase EC 3.2.1.1Bacillolysin EC 3.4.24.28Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of:Endo-1,3(4)-beta-glucanase: 2 350 U/gEndo-1,4-beta-glucanase: 4 000 U/gAlpha-amylase: 400 U/gBacillolysin: 450 U/gEndo-1,4-beta-xylanase: 20 000 U/g Turkeys for fattening — Endo-1,3(4)-beta-glucanase: 587 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. 

— endo-1,3(4)-beta-glucanase: 1 175-2 350 U,
— endo-1,4-beta-glucanase: 2 000-4 000 U,
— alpha-amylase: 200-400 U,
— bacillolysin: 225-450 U,
— endo-1,4-beta-xylanase: 10 000-20 000 U,

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 30 % wheat.
 Without a time limit
Endo-1,4-beta-glucanase: 1 000 U —
Alpha-amylase: 100 U —
Bacillolysin: 112 U —
Endo-1,4-beta-xylanase: 5 000 U —
Piglets (weaned) — Endo-1,3(4)-beta-glucanase: 1 175 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. For use in weaned piglets up to 35 kg

3. 

— endo-1,3(4)-beta-glucanase: 1 175 U,
— endo-1,4-beta-glucanase: 2 000 U,
— alpha-amylase: 200 U,
— bacillolysin: 225 U,
— endo-1,4-beta-xylanase: 10 000 U,

4. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 35 % wheat.
 
Endo-1,4-beta-glucanase: 2 000 U —
Alpha-amylase: 200 U —
Bacillolysin: 225 U —
Endo-1,4-beta-xylanase: 10 000 U —




