
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘sensory additives’ and to the functional group ‘a(ii) Colourants; substances which when fed to animals add colours to food of animal origin’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 30 April 2008.
For the Commission
Androulla VASSILIOU
Member of the Commission
ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg/kg of complete feedingstuff with a moisture content of 12 %
Category: sensory additives. Functional group: a(ii) Colourants; substances which when fed to animals add colours to food of animal origin
2a(ii) 165 — Astaxanthin dimethyldisuccinate 
 Active substance:
 Astaxanthin dimethyldisuccinate
(C50H64O10, CAS No: 578006-46-9)
 Astaxanthin dimethyldisuccinate > 96 %
 Other carotenoids < 4 %
 Additive composition:
Formulated in an organic matrix
 Purity criteria:
 Triphenylphosphine oxide (TPPO) ≤ 100 mg/kg of additive
 Dichloromethane: ≤ 600 mg/kg additive
 Analytical methods:
High performance liquid chromatography (HPLC) coupled with ultraviolet detection (UV) Salmon and trout — — 138 
1. Use permitted from the age of 6 months onwards or from 50 g weight onwards.

2. In order to be used in fish feed, the additive shall be used in a formulated form, properly stabilized with authorized antioxidants. If ethoxyquin is used in the formulation, the ethoxyquin content shall be indicated in the label.

3. If astaxanthin dimethyldisuccinate is mixed with canthaxanthin and other astaxanthin sources, the total concentration of the mixture shall not exceed 100 g astaxanthin-equivalents/kg in the complete fish feedingstuff.
 21 May 2018

