
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 15 May 2007.
For the Commission
Markos KYPRIANOU
Member of the Commission
ANNEX

Identification number of the additive Name of the holder of authorisation Additive(Trade name) Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
mg of additive/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a2 Trouw Nutrition BV Fermentation product of Aspergillus oryzaeNRRL 458(Amaferm) 
 Additive composition:
Fermentation product of Aspergillus oryzae NRRL 458: 4 to 5 %
Wheat bran: 94 to 95 %
Stainless grit containing 5 % cobalt carbonate 1 %
 Characterisation of the active substance:
Fermentation product of Aspergillus oryzae (NRRL 458) containing:
Endo-1,4-beta-glucanase EC 3.2.1.4: 3 IU/g;
Alpha-amylase EC 3.2.1.1: 40 IU/g.
 Analytical method:
Alpha amylase AOAC 17th Ed. 2002.01
Endo-1,4-beta-glucanase (based on supernatant protein and cellulase activities of the anaerobic fungus (Neocallimastix frontalis EB 188) (Barichievich, EB, Calza RE (1990)). Dairy cows — 85 300 
1. Recommended dose: the quantity of the additive in daily ration should be 3 to 5 g/cow/day.

2. For user safety: shall be used breathing protection during handling and safety glasses shall be worn.
 5 June 2017

