
Article 1 
The preparation belonging to the group ‘Enzymes’, as specified in the Annex, is authorised provisionally for four years as an additive in animal nutrition under the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 1 October 2007.
For the Commission
Markos KYPRIANOU
Member of the Commission
ANNEX

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Unit of activity/kg of complete feedingstuff
Enzymes
53 Endo-1,3(4)-beta-glucanase EC 3.2.1.6Endo-1,4-beta-glucanase EC 3.2.1.4Alpha-amylase EC 3.2.1.1Bacillolysin EC 3.4.24.28Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of:
 Endo-1,3(4)-beta-glucanase: 2 350 U/g
 Endo-1,4-beta-glucanase: 4 000 U/g
 Alpha-amylase: 400 U/g
 Bacillolysin: 450 U/g
 Endo-1,4-beta-xylanase: 20 000 U/g Laying hens — Endo-1,3(4)-beta-glucanase: 587Endo-1,4-beta-glucanase: 1 000 UAlpha-amylase: 100 UBacillolysin: 112 UEndo-1,4-beta-xylanase: 5 000 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. 

— endo-1,3(4)-beta-glucanase: 587-2 350 U
— endo-1,4-beta-glucanase: 1 000-4 000 U
— alpha-amylase: 100-400 U
— bacillolysin: 112-450 U
— endo-1,4-beta-xylanase: 5 000-20 000 U

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 30 % of wheat.
 22 October 2011




