
Article 1 
This Decision lays down detailed rules for monitoring antimicrobial resistance in accordance with Article 7(3) and Annex II(B) of Directive 2003/99/EC to be carried out in the Member States. It shall cover Salmonella spp. in fowl (Gallus gallus), turkeys, and slaughter pigs without prejudice to additional antimicrobial resistance monitoring in accordance with the requirements in Article 7(1) of Directive 2003/99/EC.
Article 2 
The collection of isolates of Salmonella spp. referred to in Article 1 and the analysis thereof shall be performed by the competent authority or under its supervision in accordance with the technical specifications set out in the Annex.
Article 3 
National aggregated data and results of the analyses shall be made available publicly in a form that ensures confidentiality.
Article 4 
This Decision shall apply from 1 January 2008.
Article 5 
This Decision is addressed to the Member States.
Done at Brussels, 12 June 2007.
For the Commission
Markos KYPRIANOU
Member of the Commission
ANNEX
1. 
Salmonella isolates collected through control and monitoring programmes, set up in accordance with Article 5 of Regulation (EC) No 2160/2003 of the European Parliament and of the Council and/or Commission Decisions 2006/662/EC, 2006/668/EC, shall be collected for antimicrobial resistance monitoring in accordance with Table 1.


Year All Salmonella serovars
Laying hens Broilers Turkeys Slaughter pigs
2007   X X
2008 X   
2009 X X  
2010 X X X 
2011 X X X X
2012 X X X X



Not more than one isolate per Salmonella serovar from the same epidemiological unit per year shall be included in the monitoring. The epidemiological unit for laying hens, broilers, and turkeys is the flock. For pigs, the epidemiological unit is the holding.

2. 
The number of Salmonella isolates to be included in the antimicrobial resistance monitoring per Member State per year shall be 170 for each study population (i.e. laying hens, broilers, turkeys and slaughter pigs).

In those Member States where, in any given year, a lower number of isolates than the target sample size is available from the monitoring or control programmes, all these isolates shall be included in the antimicrobial resistance monitoring.

In those Member States where a higher number of isolates is available all isolates, or a representative random selection equal or larger than the target sample size, shall be included.

3. 
Member States shall test at least the antimicrobials that are specified in Table 2, using the cut-off values given and an appropriate concentration range to determine the susceptibility of Salmonella.

Dilution methods shall be performed according to the methods described by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the Clinical and Laboratory Standards Institute (CLSI), accepted as international reference method (ISO standard 20776-1:2006). It is recommended that the selected isolates of S. Enteritidis and S. Typhimurium are phage typed.

4. 
The results of the antimicrobial resistance monitoring shall be assessed and reported, in accordance with Article 9 of Directive 2003/99/EC, in the yearly report on trends and sources of zoonoses, zoonotic agents and antimicrobial resistance.

Without prejudice to the provisions of Annex IV of Directive 2003/99/EC the following information shall be reported for Salmonella in laying hens, broilers, turkeys and pigs:


— Origin of isolates i.e. baseline study, control programme, passive surveillance,
— number of isolates susceptibility tested,
— number of isolates found to be resistant per antimicrobial, and
— number of fully-susceptible isolates and number of isolates resistant to 1, 2, 3, 4 and > 4 antimicrobials listed in Table 2.


 Antimicrobial Cut-off value (mg/L) R >
Salmonella Cefotaxime 0,5
Nalidixic acid 16
Ciprofloxacin 0,06
Ampicillin 4
Tetracycline 8
Chloramphenicol 16
Gentamicin 2
Streptomycin 32
Trimethoprim 2
Sulphonamides 256
