
Article 1 
The preparation belonging to the group ‘Micro-organisms’, as specified in Annex I, is authorised without a time-limit as additive in animal nutrition under the conditions laid down in that Annex.
Article 2 
The preparation belonging to the group ‘Enzymes’, as specified in Annex II, is authorised without a time-limit as additive in animal nutrition under the conditions laid down in that Annex.
Article 3 
The preparations belonging to the group ‘Enzymes’, as specified in Annex III, are authorised provisionally for four years as additives in animal nutrition under the conditions laid down in that Annex.
Article 4 
This Regulation shall enter into force on the twentieth day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 14 February 2006.
For the Commission
Markos KYPRIANOU
Member of the Commission
ANNEX I


EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
CFU/kg of complete feedingstuff
Micro-organisms
‘E 1705 Enterococcus faeciumNCIMB 10415 Preparation of Enterococcus faecium containing a minimum of:
 Microencapsulated form:
1 × 1010 CFU/g additive
 Granulated form:
3,5 × 1010 CFU/g additive Piglets — 0,35 × 109 1 × 109 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. Granulated form to be used exclusively in milk replacers.

3. For use in piglets until approximately 35 kg.
 Without a time-limit’

ANNEX II


EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
‘E 1632 3-PhytaseEC 3.1.3.8 Preparation of 3-phytase produced by Trichoderma reesei (CBS 528.94) having a minimum phytase activity of:
 Solid form: 5 000 PPU/g
 Liquid form: 5 000 PPU/g Chickens for fattening — 250 PPU — 
1. In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2. 
250-750 PPU.

3. For use in compound feed containing more than 0,22 % phytin bound phosphorus.
 Without a time-limit


ANNEX III


EC No or No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
‘28 3-PhytaseEC 3.1.3.8 Preparation of 3-phytase produced by Trichoderma reesei (CBS 528.94) having a minimum phytase activity of:
 Solid form: 5 000 PPU/g
 Liquid form: 5 000 PPU/g Laying hens — 250 PPU — 
1. In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting.

2. 
250-1 000 PPU.

3. For use in compound feed containing more than 0,22 % phytin bound phosphorus.
 7.3.2010
39 
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Endo-1,4-beta-xylanase
EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:
 Endo-1,3(4)-beta-glucanase: 800 U/g
 Endo-1,4-beta-xylanase: 800 U/g Piglets (weaned) — endo-1,3(4)-beta-glucanase:400 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 400 U
 endo-1,4-beta-xylanase: 400 U.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans) e.g. containing more than 65 % barley.

4. For weaned piglets up to approximately 35 kg.
 7.3.2010
endo-1,4-beta-xylanase:400 U —
53 
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Endo-1,4-beta-glucanase
EC 3.2.1.4
 Alpha-amylase
EC 3.2.1.1
 Bacillolysin
EC 3.4.24.28
 Endo-1,4-beta-xylanase
EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of:
 Endo-1,3(4)-beta-glucanase: 2 350 U/g
 Endo-1,4-beta-glucanase: 4 000 U/g
 Alpha-amylase: 400 U/g
 Bacillolysin: 450 U/g
 Endo-1,4-beta-xylanase: 20 000 U/g Turkeys for fattening — Endo-1,3(4)-beta-glucanase:587 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 587-2 350 U
 endo-1,4-beta-glucanase: 1 000-4 000 U
 alpha-amylase: 100-400 U
 bacillolysin: 112-450 U
 endo-1,4-beta-xylanase: 5 000-20 000 U

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 30 % of wheat.
 7.3.2010
Endo-1,4-beta-glucanase:1 000 U —
Alpha-amylase:100 U —
Bacillolysin:112 U —
Endo-1,4-beta-xylanase:5 000 U —








