
Article 1 
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘gut flora stabilisers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 29 September 2006.
For the Commission
Markos KYPRIANOU
Member of the Commission
ANNEX

Identification number of the additive Name of the holder of authorisation Additive(Trade name) Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
CFU/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: gut flora stabilisers.
4b1820 Calpis Co. LtdRepresented in the Community by Orffa International Holding BV Bacillus subtilis C-3102 (DSM 15544)(Calsporin) 
 Additive composition:
Bacillus subtilis C-3102 (DSM 15544) with minimum of 1,15 × 1010 CFU/g preparation of additive (25-30 %)
Calcium carbonate (70-75 %)
 Characterisation of the active substance:
Viable spores (CFU) of Bacillus subtilis C-3102 (DSM 15544)
 Analytical method
Enumeration spread plate method using tryptone soya agar with preheat treatment of feed samples Chickens for fattening — 1 × 109 1 × 109 For user safety: breathing protection during handling and safety glassesIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.The use is permitted in feed containing the permitted coccidiostats: monensin sodium, salinomycin sodium, seduramycin sodium, lasalocid sodium, maduramycin ammonium narasin-nicarbazin, diclazuril 20 October 2016
