
Article 1 
The preparations belonging to the group ‘Enzymes’, as specified in the Annex, are authorised for use without a time limit as additives in animal nutrition under the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the third day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 31 May 2005.
For the Commission
Markos KYPRIANOU
Member of the Commission
ANNEX

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1624 
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Alpha-amylase
EC 3.2.1.1 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of:
 Endo-1,3(4)-beta-glucanase: 250 U/g
 Endo-1,4-beta-xylanase: 400 U/g
 Alpha-amylase: 1 000 U/g Piglets (weaned) — endo-1,3(4)-beta-glucanase: 250 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 250 U
 endo-1,4-beta-xylanase: 400 U
 alpha-amylase: 1 000 U.

3. For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 35 % barley.

4. For use in weaned piglets until approximately 35 kg in weight.
 Without a time limit
endo-1,4-beta-xylanase: 400 U —
alpha-amylase: 1 000 U —
E 1625 
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Alpha-amylase
EC 3.2.1.1
 Polygalacturonase
EC 3.2.1.15 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:
 Endo-1,3(4)-beta-glucanase: 150 U/g
 Endo-1,4-beta-xylanase: 4 000 U/g
 Alpha-amylase: 1 000 U/g
 Polygalacturonase: 25 U/g Piglets (weaned) — endo-1,3(4)-beta-glucanase: 150 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 150 U
 endo-1,4-beta-xylanase: 4 000 U
 alpha-amylase: 1 000 U
 polygalacturonase: 25 U.

3. For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20 % barley and 35 % wheat.

4. For use in weaned piglets until approximately 35 kg in weight.
 Without a time limit
endo-1,4-beta-xylanase: 4 000 U —
alpha-amylase: 1 000 U —
polygalactu-ronase: 25 U —
E 1626 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Subtilisin
EC 3.4.21.62 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:
 Endo-1,4-beta-xylanase: 5 000 U/g
 Subtilisin: 500 U/g Piglets (weaned) — endo-1,4-beta-xylanase: 5 000 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 5 000 U
 subtilisin: 500 U.

3. For use in compound feed e.g. containing more than 40 % wheat.

4. For use in weaned piglets until approximately 35 kg in weight.
 Without a time limit
subtilisin: 500 U —
E 1627 
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Endo-1,4-beta-xylanase
EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:
 Endo-1,3(4)-beta-glucanase: 800 U/g
 Endo-1,4-beta-xylanase: 800 U/g Pigs for fattening — endo-1,3(4)-beta-glucanase: 400 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,3(4)-beta-glucanase: 400 U
 endo-1,4-beta-xylanase: 400 U.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans) e.g. containing more than 65 % barley.
 Without a time limit
endo-1,4-beta-xylanase: 400 U —
E 1628 Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:
 Powder form: Endo-1,4-beta-xylanase: 2 000 U/g
 Liquid form: Endo-1,4-beta-xylanase: 5 000 U/ml Chickens for fattening — endo-1,4-beta-xylanase: 500 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 500-2 500 U.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 55 % wheat or 60 % rye.
 Without a time limit
E 1629 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of:
 Endo-1,4-beta-xylanase: 5 000 U/ml
 Endo-1,3(4)-beta-glucanase: 50 U/ml Chickens for fattening — endo-1,4-beta-xylanase: 1 250 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 1 250-2 500 U
 endo-1,3(4)-beta-glucanase: 12-25 U.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20 % barley and 40 % wheat.
 Without a time limit
endo-1,3(4)-beta-glucanase: 12 U —




