
Article 1 
The preparations belonging to the group ‘Micro-organisms’, as specified in Annex I, are authorised for use without a time limit as additives in animal nutrition under the conditions laid down in that Annex.
Article 2 
The preparations belonging to the group ‘Enzymes’, as specified in Annex II, are authorised for use without a time limit as additives in animal nutrition under the conditions laid down in that Annex.
Article 3 
The preparations belonging to the group ‘Enzymes’, as specified in Annex III, are authorised for use for four years as additives in animal nutrition under the conditions laid down in that Annex.
Article 4 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 14 December 2005.
For the Commission
Markos KYPRIANOU
Member of the Commission
ANNEX I

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
CFU/kg of complete feedingstuff
Micro-organisms
E 1703 Saccharomyces cerevisiaeCNCM I-1079 Preparation of Saccharomyces cerevisiae containing a minimum of:2 × 1010 CFU/g additive Sows — 1 × 109 6 × 109 In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting. Without a time limit
E 1712 Pediococcus acidilacticiCNCM MA 18/5M Preparation of Pediococcus acidilactici containing a minimum of:1 × 1010 CFU/g additive Pigs for fattening — 1 × 109 1 × 109 In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting. Without a time limit
E 1713 Enterococcus faeciumCECT 4515 Preparation of Enterococcus faecium containing a minimum of:1 × 109 CFU/g additive Piglets (weaned) — 1 × 109 1 × 109 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. For use in weaned piglets until approximately 35 kg.
 Without a time limit
ANNEX II

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1636 Endo-1,3(4)-beta-glucanaseEC 3.2.1.6 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:
 Solid form: 700 000 BU/g
 Liquid form: 300 000 BU/g Chickens for fattening — 17 500 BU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 17 500-50 000 BU.

3. For use in compound feed rich in non-starch polysaccharides (mainly glucans), e.g. containing more than 20 % barley or 30 % rye.
 Without a time limit
E 1637 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Subtilisin
EC 3.4.21.62
 Alpha-amylase
EC 3.2.1.1
 Polygalacturonase
EC 3.2.1.15 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a mimimum activity of:
 Endo-1,4-beta-xylanase: 300 U/g
 Endo-1,3(4)-beta-glucanase: 150 U/g
 Subtilisin: 4 000 U/g
 Alpha-amylase: 400 U/g
 Polygalacturonase: 25 U/g Chickens for fattening — endo-1,4-beta-xylanase:300 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 300 U
 endo-1,3(4)-beta-glucanase: 150 U
 subtilisin: 4 000 U
 alpha-amylase: 400 U
 polygalacturonase: 25 U.

3. For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize or 60 % wheat.
 Without a time limit
endo-1,3(4)-beta-glucanase:150 U —
subtilisin:4 000 U —
alpha-amylase:400 U —
polygalacturonase:25 U —






ANNEX III

EC No or No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
5 Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Aspergillus oryzae (DSM 10287) having a minimum activity of:
 Coated form:
1 000 FXU/g
 Liquid form:
650 FXU/ml Pigs for fattening — 200 FXU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 200-400 FXU.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % cereals (e.g. wheat, barley, rye or triticale).
 4.1.2010
Ducks — 100 FXU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 100-200 FXU.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % cereals (e.g. wheat, barley, rye or triticale).
 4.1.2010
37 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Subtilisin
EC 3.4.21.62 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:
 Endo-1,4-beta-xylanase: 5 000 U/g
 Subtilisin: 1 600 U/g Ducks — endo-1,4-beta-xylanase:2 500 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 2 500 U
 subtilisin: 800 U.

3. For use in compound feed e.g. containing more than 65 % wheat.
 4.1.2010
subtilisin:800 U —
59 
 Endo-1,4-beta-xylanase
EC 3.2.1.8
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Subtilisin
EC 3.4.21.62
 Alpha-amylase
EC 3.2.1.1
 Polygalacturonase
EC 3.2.1.15 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a mimimum activity of:
 Endo-1,4-beta-xylanase: 300 U/g
 Endo-1,3(4)-beta-glucanase: 150 U/g
 Subtilisin: 4 000 U/g
 Alpha-amylase: 400 U/g
 Polygalacturonase: 25 U/g Ducks — endo-1,4-beta-xylanase:300 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 300 U
 endo-1,3(4)-beta-glucanase: 150 U
 subtilisin: 4 000 U
 alpha-amylase: 400 U
 polygalacturonase: 25 U.

3. For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize.
 4.1.2010
endo-1,3(4)-beta-glucanase:150 U —
subtilisin:4 000 U —
alpha-amylase:400 U —
polygalacturonase:25 U —
Laying hens — endo-1,4-beta-xylanase:225 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2. 

 endo-1,4-beta-xylanase: 225 U
 endo-1,3(4)-beta-glucanase: 112 U
 subtilisin: 3 000 U
 alpha-amylase: 300 U
 polygalacturonase: 18 U.

3. For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize.
 4.1.2010
endo-1,3(4)-beta-glucanase:112 U —
subtilisin:3 000 U —
alpha-amylase:300 U —
polygalac-turonase:18 U —





