
Article 1 
The preparation belonging to the group ‘Enzymes’, as specified in Annex I, is authorised for use without a time limit as additive in animal nutrition under the conditions laid down in that Annex.
Article 2 
The preparations belonging to the group ‘Enzymes’, as specified in Annex II, are authorised for use for four years as additive in animal nutrition under the conditions laid down in that Annex.
Article 3 
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 8 September 2005.
For the Commission
Markos KYPRIANOU
Member of the Commission
ANNEX I

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1634 Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus niger (MUCL 39199) having a minimum activity of:
 Solid form:
1 500 AGL/g
 Liquid form:
200 AGL/ml Chickens for fattening — 25 AGL — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. 

 25-100 AGL

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 50 % barley
 Without a time limit

ANNEX II

No or EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
54 Endo-1,3(4)-beta-glucanase EC 3.2.1.6Endo-1,4-beta-glucanase EC 3.2.1.4Alpha-amylase EC 3.2.1.1Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of:
 Endo-1,3(4)-beta-glucanase: 10 000 U/g
 Endo-1,4-beta-glucanase: 120 000 U/g
 Alpha-amylase: 400 U/g
 Endo-1,4-beta-xylanase: 210 000 U/g Laying hens — Endo-1,3(4)-beta-glucanase: 500 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. 

 endo-1,3(4)-beta-glucanase: 500-1 500 U
 endo-1,4-beta-glucanase: 6 000-18 000 U
 alpha-amylase: 20-60 U
 endo-1,4-beta-xylanase: 10 500-31 500 U

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30 % wheat
 29.9.2009
endo-1,4-beta-glucanase: 6 000 U —
alpha-amylase: 20 U —
endo-1,4-beta-xylanase: 10 500 U —
60 Endo-1,4-beta-xylanase EC 3.2.1.8Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of:Liquid form:
 Endo-1,4-beta-xylanase: 5 000 U/ml
 Endo-1,3(4)-beta-glucanase: 50 U/ml Turkeys for fattening — Endo-1,4-beta-xylanase: 1 250 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelletting

2. 

 endo-1,4-beta-xylanase: 1 250-2 500 U
 endo-1,3(4)-beta-glucanase: 12-25 U

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20 % barley and 40 % wheat
 29.9.2009
endo-1,3(4)-beta-glucanase: 12 U —
62 Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) having a minimum activity of:
 Solid form:
5 600 TXU/g
 Liquid form
5 600 TXU/ml Chickens for fattening — 200 TXU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelletting

2. Recommended dose per kg of complete feedingstuff: 400-800 TXU

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40 % wheat
 29.9.2009






