
Article 1 
The preparations belonging to the group ‘Enzymes’, as specified in the Annex, are authorised for use without a time-limit as additives in animal nutrition under the conditions laid down in that Annex.
Article 2 
This Regulation shall enter into force on the third day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.Done at Brussels, 27 July 2005.
For the Commission
Neelie KROES
Member of the Commission
ANNEX

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1602 Endo-1,4-beta-glucanaseEC 3.2.1.4Endo-1,3(4)-beta-glucanaseEC 3.2.1.6Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-glucanase, endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 74 252) having a minimum activity of:
 liquid and granular form:
 endo-1,4-beta-glucanase: 8 000 U/ml or g
 endo-1,3(4)-beta-glucanase: 18 000 U/ml or g
 endo-1,4-beta-xylanase: 26 000 U/ml or g Turkeys for fattening — Endo-1,4-beta-glucanase: 400 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. 

 endo-1,4-beta-glucanase: 400-800 U
 endo-1,3(4)-beta-glucanase: 900-1 800 U
 endo-1,4-beta-xylanase: 1 300-2 600 U

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 25 % wheat or 20 % barley and 5 % rye
 Without a time-limit
Endo-1,3(4)-beta-glucanase: 900 U —
Endo-1,4-beta-xylanase: 1 300 U —
E 1604 Endo-1,3(4)-beta-glucanaseEC 3.2.1.6Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced from Penicillium funiculosum (IMI SD101) having a minimum activity of:
 powder form:
 endo-1,3(4)-beta-glucanase: 2 000 U/g
 endo-1,4-beta-xylanase: 1 400 U/g
 liquid form:
 endo-1,3(4)-beta-glucanase: 500 U/ml
 endo-1,4-beta-xylanase: 350 U/ml Pigs for fattening — Endo-1,3(4)-beta-glucanase: 100 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. 

 endo-1,3(4)-beta-glucanase: 100 U
 endo-1,4-beta-xylanase: 70 U

3. For use in compound feed rich in non-starch polysaccharides (mainly beta glucans and arabinoxylans), e.g. containing more than 40 % barley or 20 % wheat
 Without a time-limit
Endo-1,4-beta-xylanase: 70 U —
E 1606 Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG S-15136) having a minimum activity of:
 solid and liquid forms:
 100 IU/g or ml Piglets (weaned) — 10 IU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. 

 endo-1,4-beta-xylanase: 10 IU

3. For use in compound feed rich in arabinoxylan, e.g. minimum 40 % wheat or barley

4. For use in weaned piglets up to approximately 35 kg
 Without a time-limit
E 1633 Endo-1,3(4)-beta-glucanaseEC 3.2.1.6Endo-1,4-beta-xylanaseEC 3.2.1.8SubtilisinEC 3.4.21.62 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:
 solid form:
 endo-1,3(4)-beta-glucanase: 100 U/g
 endo-1,4-beta-xylanase: 300 U/g
 subtilisin: 800 U/g Chickens for fattening — Endo-1,3(4)-beta-glucanase: 30 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting

2. 

 endo-1,3(4)-beta-glucanase: 30-100 U
 endo-1,4-beta-xylanase: 90-300 U
 subtilisin: 240-800 U

3. For use in compound feed, e.g. containing more than 60 % barley
 Without a time-limit
Endo-1,4-beta-xylanase: 90 U —
Subtilisin: 240 U —








