
Article 1 
The preparation belonging to the group ‘micro-organisms’ as set out in Annex I is authorised for use as additive in animal nutrition under the conditions laid down in that Annex.
Article 2 
The preparations belonging to the group ‘enzymes’ as set out in Annexes II, III, IV, V and VI are authorised for use as additive in animal nutrition under the conditions laid down in those Annexes.
Article 3 
This Regulation shall enter into force on the third day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member StatesDone at Brussels, 8 July 2004.
For the Commission
David BYRNE
Member of the Commission
ANNEX I

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
CFU/kg of complete feedingstuff
Micro-organisms
E 1707 Enterococcus faeciumDSM 10663/NCIMB 10415 Preparation of Enterococcus faecium containing a minimum of:
 powder and granulated form:
3,5 × 1010 CFU/g additive
 coated form:
2,0 × 1010 CFU/g additive
 liquid form:
1 × 1010 CFU/ml additive Chickens for fattening — 1 × 109 1 × 109 In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelletingMay be used in compound feed containing the permitted coccidiostats: diclazuril, halofuginone, lasalocid sodium, maduramicin ammonium, monensin sodium, robenidine Without a time limit
ANNEX II

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1602 
 Endo-1,4-beta-glucanase
EC 3.2.1.4
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Endo-1,4-beta-xylanase
EC 3.2.1.8 Preparation of endo-1,4-beta-glucanase, endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 74 252) having a minimum activity of:Liquid and granular form:
 Endo-1,4-beta-glucanase:
8 000 U/ml or g
 Endo-1,3(4)-beta-glucanase:
18 000 U/ml or g
 Endo-1,4-beta-xylanase:
26 000 U/ml or g Chickens for fattening — endo-1,4-beta-glucanase:400 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. 

 endo-1,4-beta-glucanase:
400-1 600 U
 endo-1,3(4)-beta-glucanase:
900-3 600 U
 endo-1,4-beta-xylanase:
1 300-5 200 U

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30 % wheat or barley and more than 10 % rye
 Without a time limit
endo-1,3(4)-beta-glucanase:900 U —
endo-1,4-beta-xylanase:1 300 U —



ANNEX III

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1603 Endo-1,3(4)-beta-glucanaseEC 3.2.1.6 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:
 coated form:
50 FBG/g
 liquid form:
120 FBG/ml Chickens for fattening — 10 FBG — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. Recommended dose per kg of complete feedingstuff: 15-20 FBG

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 60 % vegetable ingredients (maize, lupin, wheat, barley, soya, rice, oilseed rape or peas)
 Without a time limit

ANNEX IV

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1604 
 Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
 Endo-1,4-beta-xylanase
EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Penicillium funiculosum (IMI SD 101) having a minimum activity of:
 powder form:
 endo-1,3(4)-beta-glucanase:
 2 000 U/g
 endo-1,4-beta-xylanase:
1 400 U/g
 liquid form:
 endo-1,3(4)-beta-glucanase:
 500 U/ml
 endo-1,4-beta-xylanase
350 U/ml Chickens for fattening — 
 endo-1,3(4)-beta-glucanase:
 100 U
 endo-1,4-beta-xylanase:
70 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. 

 endo-1,3(4)-beta-glucanase: 100 U
 endo-1,4-beta-xylanase: 70 U

3. For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucan and arabinoxylans), e.g. containing more than 50 % barley or 60 % wheat
 Without a time limit


ANNEX V

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1605 Endo-1,4-beta-xylanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 520.94) having a minimum activity of:
 solid form:
Endo-1,4-beta-xylanase:
600 U/g
 liquid form:
Endo-1,4-beta-xylanase:
300 U/ml Chickens for fattening — 300 U — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2. Recommended dose per kg of complete feedingstuff: endo-1,4-beta-xylanase: 300-600 U

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % wheat
 Without a time limit

ANNEX VI

EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1606 Endo-1,4-beta-xilanaseEC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG-S 15136) having a minimum activity of:
 Solid and liquid form:
100 IU/g or ml Chickens for fattening — 10 IU — 
1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting

2. Recommended dose per kg of complete feedingstuff: 10 IU

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat
 Without a time limit
